Fig. 5
From: Polycomb contraction differentially regulates terminal human hematopoietic differentiation programs
BMI1 is expressed at a lower level in monocytes, erythroblasts and ESCs compared to other cell types. A Expression (RPKM) of PRC1, PRC2, and H3K27me3 demethylase members across cell types with broad (label as broad) and punctate (labeled as punctate) H3K27me3 profile. Cell types are indicated by the color legend. B Volcano plot of expression (RPKM) of PRC1, PRC2, and H3K27me3 demethylase members across cell types with broad and punctate H3K27me3 profile. C Expression (TPM) of Bmi1 in mouse hematopoietic cells. D Global measurement of H3, H2AK119Ub, H3K9me3, and H3K27me3 by western blot across BMI1-wild type (wt) and BMI1-knockout (KO). E Total number of cells at 3 days post CRISPR treatment across BMI1-wt and KO (left panel). Percentage of viable cells (right panel) (N=6) (two-sided t-test *p < 0.05, **p < 0.01, ***p < 0.001)