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Fig. 5 | BMC Biology

Fig. 5

From: Ileum tissue single-cell mRNA sequencing elucidates the cellular architecture of pathophysiological changes associated with weaning in piglets

Fig. 5

Weaning induced ileum inflammation and mitochondrial damage in epithelial cells. a Scanning electron microscope of the ileum tissue from NSP and PWP (left panel); H&E staining (right panel). b Boxplots depicting mean gene expression of inflammatory signatures from NSPs and PWPs, ***p<0.001; error bars: SEM; Wilcoxon test. c TUNEL staining in ileum tissues from NSP and PWP. The nuclei of apoptotic cells are stained with green. Scale bars, 500 μm. d Scatterplots showing differential gene expression in enterocytes between PWPs and NSPs. Genes with p value<0.05 and log2 (FC)>0.25 are shown. e Heatmap shows the log2 (FC) in gene expression of BCL2, BAX, and BH3-only family genes between NSPs and PWPs. *p<0.05, **p<0.01, ***p<0.001, Wilcoxon test. f IHC images of ileum tissue from NSPs and PWPs stained for BID (n=6). Scale bars, 20 μm. g TEM analysis shows mitochondrial ultrastructure of enterocytes (n=6). Red arrows indicate damaged mitochondria with disrupted respiratory cristae. Scale bars, 1 μm

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