Fig. 7
Cytokine-regulated plasticity of Th17 cells toward a pro-inflammation signature in the piglet ileum. a GO analysis of dendritic cell (DC) cluster-derived differentially expressed genes. Selected GO terms with Benjamini-Hochberg-corrected p values<0.05 (one-sided Fisher’s exact test). b Volcano plots for differentially expressed genes between DCs (left) and Th17 cells (right) in PWPs relative to NSPs. Hurdle model likelihood ratio test for differential expression, Benjamini–Hochberg multiple testing correction. All genes given in Additional file 18: Table S9. c IF staining showing the increased abundance of IL1β (red) and TNF-α (green) in PWPs compared with NSPs (n=6). Left: images of IL1β (red) and TNF-α (green) antibody staining; scale bars, 100 μm. Right: number of colored dots in the field of view (mm2) (n=6); **p<0.01, ***p<0.001; error bars: SEM; Student’s t-test. d Violin plot showing the gene expression levels relating to memory and tissue-resident phenotypes in Th17 cells from NSPs and PWPs. *p<0.05, **p<0.01, ***p<0.001, Wilcoxon test. e Violin plot comparing cytokine gene expression levels in T cell subpopulations. The specific cell types that express target cytokines are given in Additional file 19: Fig. S10f. *p<0.05, **p<0.01, ***p<0.001, Wilcoxon test. f ELISA showing changes in porcine cytokine production. *p<0.05, mean ± SEM (n=6), Student’s t-test. j Schematic drawing showing Th17 plasticity-regulated changes in cytokine environment