Fig. 6

Confirmation of ortho-cleavage activity of T. urticae DOGs. A Chemical structures of the monocyclic and polycyclic catecholic compounds subjected to UPLC-MS. The ortho-cleavage positions are indicated with a red thunderbolt sign. B Percentage metabolized substrate as estimated from residual substrate in active and inactive enzyme treatments after 20 min incubation at 25 °C. The enzyme used with the various substrates is indicated under the curve. Error bars represent the standard error of the mean (n = 5)