Intradiol ring cleavage dioxygenases from herbivorous spider mites as a new detoxification enzyme family in animals

Table 1 Steady state kinetic parameters (25 °C) of recombinant T. urticae DOG enzymes with catechol, 4-methyl catechol, and 4-chlorocatechol

DOG	Substrate	K_M (μM)	V_max (μM min⁻¹)	k_cat (min⁻¹)	k_cat/K_M (μM⁻¹ min⁻¹)
TuDOG1	Catechol	15.2 ± 4.5	0.14 ± 0.01	2.269 ± 0.162	0.149 ± 0.036
	4-Methyl catechol	15.1 ± 2.7	0.12 ± 0.01	1.945 ± 0.162	0.129 ± 0.060
	4-Chlorocatechol	21.2 ± 7.4	0.08 ± 0.01	1.297 ± 0.162	0.061 ± 0.022
TuDOG7	Catechol	74.9 ± 30.9	0.42 ± 0.06	1.340 ± 0.191	0.018 ± 0.006
	4-Methyl catechol	62.2 ± 42.3	0.53 ± 0.23	1.691 ± 0.734	0.027 ± 0.017
	4-Chlorocatechol	115.2 ± 35.7	0.96 ± 0.23	3.062 ± 0.734	0.027 ± 0.021
TuDOG11	Catechol	64.9 ± 24.3	0.56 ± 0.04	3.202 ± 0.229	0.049 ± 0.009
	4-Methyl catechol	153.9 ± 24.6	0.58 ± 0.04	3.316 ± 0.229	0.022 ± 0.009
	4-Chlorocatechol	25.2 ± 4.6	0.09 ± 0.01	0.515 ± 0.057	0.020 ± 0.012
TuDOG15	Catechol	91.0 ± 21.2	0.09 ± 0.01	0.797 ± 0.089	0.009 ± 0.004
	4-Methyl catechol	117.4 ± 46.5	1.77 ± 0.37	15.678 ± 3.277	0.134 ± 0.070
	4-Chlorocatechol	72.0 ± 21.3	0.62 ± 0.09	5.492 ± 0.797	0.076 ± 0.037
TuDOG16	Catechol	51.0 ± 23.4	0.06 ± 0.01	0.483 ± 0.081	0.009 ± 0.003
	4-Methyl catechol	52.4 ± 19.6	0.14 ± 0.02	1.127 ± 0.161	0.022 ± 0.008
	4-Chlorocatechol	154.3 ± 20.9	0.31 ± 0.01	2.496 ± 0.081	0.016 ± 0.004

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