Fig. 3
From: The H3K27M mutation alters stem cell growth, epigenetic regulation, and differentiation potential
H3K27M hESCs undergoing undirected differentiation exhibit altered growth and increased expression of mesodermal and ectodermal genes. A Scheme for undirected differentiation in KOSR media and Scorecard analysis. B Representative bright field images of the WT and H3K27M models at day 3 of differentiation in KOSR (scale bar=100μm). C, D hESC Scorecard assays were performed on six independent biological replicate samples encompassing three different clonal lines carrying the H3K27M mutation (Additional file 2: Table S1), with gene expression fold changes relative to the reference standard indicated for each marker indicated in C and general expression trends for each category of markers shown in D. E Representative immunostaining images for the indicated proteins in RCAS DMG tumor tissue sections are shown. Scale bar=50μm