Fig. 5

Oligodendrocyte types in spinal cord gray matter (GM) and white matter (WM). a Maps of 124 genes (left) and 13 cell types (middle and right) in the spinal cord sections (P60) targeted by in situ sequencing. The dashed line in the gene map highlights the separation between gray matter (GM) and white matter (WM). The scale bar is 100 μm. b Heatmaps of the density of cells for each oligodendrocyte type, normalized by the maximum density over all types. The scale bar is 100 μm. c Change of relative abundances of oligodendrocytes between GM and WM for P20 and P60 (left) as well as change of relative abundances of oligodendrocytes between P20 and P60 for GM and WM (right) (n = 6 P20 tissue sections, n = 6 P60 tissue sections). Statistical comparisons were determined using ANOVA and post hoc test with Tukey correction (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001)