Skip to main content
Fig. 6 | BMC Biology

Fig. 6

From: Elevated testicular apoptosis is associated with elevated sphingosine driven by gut microbiota in prediabetic sheep

Fig. 6

Fecal microbiota transplantation to confirm the influence of microbiota dysbiosis on spermatogenesis. A H&E staining of control-FMT, prediabetes-FMT, and rescue-FMT testicular tissue. B Percentage of normal seminiferous tubules in testes of the three groups (n = 3). C IHC staining for MVH in representative sections of the testicular tissues of the three groups. D The rate of MVH-positive cells within seminiferous tubules in testes of the three groups (n = 9). E (a) Western blot of MVH in the three FMT groups. (b) Relative expression of MVH in each group (n = 4). F The relative abundance of (a) sphingosine in serum and (b) testes of the three FMT groups (n = 6). G (a) TUNEL staining of representative sections within testicular and epididymal tissues of three FMT groups, and the rate of TUNEL-positive cells in (b) testicular tubules (n = 3) and (c) epididymides (n = 3). H The relative expression of (a) Bcl-2, (b) Bax, (c) Bax/Bcl-2, and (d) Caspase3 in testes of the three FMT groups (n = 3). I (a) Western blot of Actived-CASPASE-3, Pro-CASPASE-3, BCL-2, BAX, and β-ACTIN in the three FMT groups; the relative expression of (b) BAX/BCL-2 and (c) Actived-CASPASE-3/Pro-CASPASE-3 in the three FMT groups (n = 3)

Back to article page