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Fig. 1 | BMC Biology

Fig. 1

From: Inherent constraints on a polyfunctional tissue lead to a reproduction-immunity tradeoff

Fig. 1

Single-nucleus sequencing of Drosophila fat body tissue. A Cox proportional hazard analysis showed that the mated Drosophila melanogaster females have significantly lower survival than virgin females (n = 40; p = 0.0001) after infection with the Gram-negative bacterium Providencia rettgeri. Survival of uninfected virgin and mated females was not different over four days. B Combined Uniform Manifold Approximation and Projection (UMAP) of 56,000 nuclei from two replicates each of Virgin-Uninfected, Virgin-Infected, Mated-Uninfected, and Mated-Infected colored by their treatment identity. Clusters 0, 1, 2, 5, 7, 10, 11, 12, 13, and 16 marked with an asterisk (*) represent subpopulations of the fat body tissue. C Percentage distribution of nuclei from four treatments (Virgin-Uninfected, Virgin-Infected, Mated-Uninfected, and Mated-Infected) across 19 clusters. All clusters are present in constant proportion across all four treatments. D Dot Plot showing expression of marker genes per cluster for the eleven largest clusters. Average scaled expression of a marker gene across all cells is represented by the color gradient and dot size represents the percentage of cells that express the marker in each cluster. The cluster labels above the figure indicate the three genes (x-axis) that are most strongly associated with that cluster. Genes hsp27 and wisp are also top markers for Cluster 9 (see Additional file 1 for more information on cluster properties and markers)

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