Fig. 8From: A comparative meta-proteomic pipeline for the identification of plasmodesmata proteins and regulatory conditions in diverse plant speciesEctopic Medtr1g073320 expression affects rhizobia infection and callose regulation in nitrate replete conditions. Medicago truncatula roots expressing an empty vector (control) or p35S-Medtr1g073320 were grown in 0 (no nitrate) or 5 mM nitrate and inoculated with either a mock or rhizobia (infected) liquid culture. Graphs show infection thread density (a) and nodule number (b) at 7 days post- rhizobia inoculation. Different letters refer to statistically significant differences (p <0.05) according to Student’s T-test (see also Additional file 2: Fig. S9). c-g Immunofluorescent localization of callose was carried out in p35S-Medtr1g073320 roots grown in no nitrate or nitrate replete conditions and inoculated with either a mock or rhizobia culture (infected). Roots were fixed 24 hr post inoculation and callose antibodies were detected with Alexa 488 conjugated antibody (green). Panels c-f show representative confocal pictures of each condition. The vascular cylinder (vasc) and the epidermis (ep) are indicated. Scale bars= 50 μm. g Integrated density was calculated for each image in a region of interest (ROI) of approximately 100 μm2 in at least 3 biological repetitions. Boxes delimit minimum to maximum integrated density values. The central lines refer to the mean. Different letters refer to statistically significant differences (p < 0.05, Student’s T-test)Back to article page