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Fig. 8 | BMC Biology

Fig. 8

From: A comparative meta-proteomic pipeline for the identification of plasmodesmata proteins and regulatory conditions in diverse plant species

Fig. 8

Ectopic Medtr1g073320 expression affects rhizobia infection and callose regulation in nitrate replete conditions. Medicago truncatula roots expressing an empty vector (control) or p35S-Medtr1g073320 were grown in 0 (no nitrate) or 5 mM nitrate and inoculated with either a mock or rhizobia (infected) liquid culture. Graphs show infection thread density (a) and nodule number (b) at 7 days post- rhizobia inoculation. Different letters refer to statistically significant differences (p <0.05) according to Student’s T-test (see also Additional file 2: Fig. S9). c-g Immunofluorescent localization of callose was carried out in p35S-Medtr1g073320 roots grown in no nitrate or nitrate replete conditions and inoculated with either a mock or rhizobia culture (infected). Roots were fixed 24 hr post inoculation and callose antibodies were detected with Alexa 488 conjugated antibody (green). Panels c-f show representative confocal pictures of each condition. The vascular cylinder (vasc) and the epidermis (ep) are indicated. Scale bars= 50 μm. g Integrated density was calculated for each image in a region of interest (ROI) of approximately 100 μm2 in at least 3 biological repetitions. Boxes delimit minimum to maximum integrated density values. The central lines refer to the mean. Different letters refer to statistically significant differences (p < 0.05, Student’s T-test)

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