Fig. 2

CG4562 is heme-inducible and knocking down in the whole body and the gut resulted in growth defects. A Endogenous expressions of the MRP genes in S2 cell without hemin treatment. Expressions quantified by real-time relative RT-PCR normalized to rp49. All values are available in Additional file 3: Fig. 2A. B Transcription level changes of the MRP genes induced by 100 μM heme in S2 cell. Expressions of MRP genes after 4 h of heme induction were quantified by qPCR normalized by rp49. Expression values are relative to those with DMSO treatment. All values are available in Additional file 3: Fig. 2B. C Endogenous expressions of the MRP genes in the gut of the 3rd instar larva quantified by relative PCR normalized to rp49. All values are available in Additional file 3: Fig. 2C. D Relative transcription level changes of the MRP genes in the gut when supplementing 1 mM hemin in the food. Expressions quantified by relative PCR normalized to rp49. Expression is relative to that with DMSO treatment. All values are available in Additional file 3: Fig. 2D. E Phenotypic analyses of the MRP genes after knocking down in the whole body by Actin-GAL4 directed RNAi. Eclosion rate normalized to the control. All values are available in Additional file 3: Fig. 2E. F Phenotypic analyses of the MRP genes after knocking down in the gut by NP3084-GAL4-directed RNAi. All values are available in Additional file 3: Fig. 2F. Data are presented as means ± SD by two-tailed unpaired Student’s t test. *p < 0.05, **p < 0.01; ***p < 0.001. Experiments were repeated at least two times, and data from only one representative experiment were shown. The RNAi lines and their phenotype summary of this screening were attached in Additional file 1: Table S1