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Fig. 3 | BMC Biology

Fig. 3

From: Identification and characterization of a heme exporter from the MRP family in Drosophila melanogaster

Fig. 3

Characterization of some other potential Drosophila heme transporters (non-MRP transporters). A A list of other potential heme transporters in Drosophila. B Localization of these potential heme transporters when overexpressed in fusion with GFP in S2 cells. Scale bar = 10 nm. C Quantitative measurement of ZnMP fluorescence of these potential Drosophila heme transporters when overexpressed in S2 cells. All values are available in Additional file 3: Fig. 3C. D, E Phenotypic analyses of these potential heme transporters when knocking down in the whole body (Actin-GAL4) (D) and the gut (NP3084-GAL4) (E). Eclosion rate normalized to the control. All values are available in Additional file 3: Fig. 3D and Fig. 3E. Data are presented as means ± SD by two-tailed unpaired Student’s t test. *p < 0.05, **p < 0.01; ***p < 0.001. The experiments were repeated at least two times, and only one representative one was shown. The RNAi lines and their phenotype summary of this screening were attached in Additional file 1: Table S1

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