Fig. 3

Engineered truncation of up to 60 amino acid residues from C-terminus improved GsCelA activity and thermostability. a Enzyme activities at different temperatures with CMC or swollen Avicel used as substrate in 50 mM sodium acetate, pH 5. b Thermostability assays with samples pre-incubated at different temperatures. Data are mean ± SD. One enzyme unit (U) is defined as 1 μmole reducing sugar, i.e., equivalent to glucose, produced per minute. All experiments were performed in triplicate