Fig. 5

GsCelA self-truncation still takes place with different C-terminal mutants. a The truncation process was assayed with GsCelA mutants carrying partial deletion at C-terminus after incubation at 25 °C for 120 h. b Δ339-368 mutant with 30 amino acids near the C-terminus removed was still capable of truncation. c Single mutation of K315A, the truncation site, did not affect the truncation process after incubation at 25 °C for 120 h. Labels of “+” and “−” indicate samples with or without self-truncation, respectively. Full-length and truncated GsCelA, which correspond to samples before and after incubation, were used as positive and negative controls, respectively. Marker and protein profiles in b and c were cut from the same SDS-PAGE gel