Fig. 8

GsCelA truncation process is inhibited by metal chelators but can be recovered with divalent metal ions. a FL-GsCelA was incubated in the presence of different protease inhibitors, EDTA and EGTA, at 25 °C for 120 h. EDTA-pretreated GsCelA (i.e., full length) was a negative control. b Purified GsCelA does not have general protease activity. GsCelA was co-incubated with BSA at 25 °C for 120 h. Note that BSA did not get degraded yet GsCelA showed self-truncation. Lane 1: protein marker; lane 2: GsCelA pretreated with 10 mM EDTA; lane 3: self-truncated GsCelA; lane 4: BSA only; lane 5 and 6: BSA co-incubated with GsCelA for 0 and 120 h. c Effect of presence of divalent ions (i.e., Mg²⁺, Ca²⁺, Cu²⁺, Mn²⁺, or Co²⁺) and monovalent ions (i.e., Li⁺, Na⁺ or K⁺) on recovering GsCelA’s truncation ability. Different metal ions were added to EDTA-pretreated GsCelA to a final concentration of 10 mM, and the incubation was performed at 25 a for 120 h