Fig. 1
Specific depletion of Mettl14 in retinal rods led to impaired visual function. A Immunoblotting and quantitative comparison of METTL14 protein expression in retinas from Mettl14flox/flox (Ctrl) and Mettl14flox/flox; Rho-Cre (RKO) mice (n = 6). GAPDH was used as a loading control. B Immunofluorescence labeling of retinal cryosections from Ctrl and RKO mice at the age of 2 months using a METTL14 antibody (green). DAPI (blue) was used to counterstain the nuclei. Arrowheads indicated that METTL14 is still expressed in cone cells in RKO retinas. Scale bar: 25 μm. C Representative electroretinogram (ERG) traces corresponding to responses elicited by scotopic conditions at flash intensities of 0.03, 0.3, 3, and 20 cd·sec/m.2 in mice at 5 months of age. D, E Statistical analysis was performed for the amplitudes of the a-wave (D) and b-wave (E) under scotopic conditions (n = 8, two-way ANOVA followed by Tukey’s post hoc test). ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. *p < 0.05; **p < 0.01; ***p < 0.001. #, no significant difference. Data are presented as the mean ± SEM