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Fig. 5 | BMC Biology

Fig. 5

From: Mettl14-mediated m6A modification is essential for visual function and retinal photoreceptor survival

Fig. 5

Characterization of m6A modification and gene expression changes in RKO retinas. A Top consensus motif identified via HOMER with m6A-seq in Ctrl and RKO retinas. B Number of m6A peaks identified by m6A-seq in Ctrl and RKO retinas. C Number of m6A-modified genes identified by m6A-seq. Common m6A genes contain at least 1 common m6A peak, while unique m6A genes contain no common m6A peaks. D Distribution of m6A peaks in transcript segments divided into the 5′ UTR, CDS, and 3′ UTR in Ctrl and RKO retinas. Enlarged images of the boxed area are shown in the right panel. E Graphs of the m6A peak distribution showing the proportion of total m6A peaks in the indicated regions across the entire set of mRNA transcripts. F Volcano plots displaying the mRNAs that were differentially expressed between the RKO and Ctrl groups and their statistical significance (fold changes ≥ 1.2 and p < 0.05). G Venn diagram showing the numbers of shared genes with reduced m6A modification (m6A downregulation) identified by m6A-seq among genes with downregulated expression identified by RNA-seq. H, I Pathway analysis (H) and significantly enriched (p-value < 0.01, correction with Benjamini–Hochberg multiple testing) GO terms in cellular component (I) categories for 224 genes selected in Fig. 5G

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