Fig. 3

Increased intracellular pH enhances glycolysis in breast cancer cells. A Increased intracellular pH, using gluconate substituted media, increases the glucose-induced PPR in MCF7 cells irrespective of pHe. N = 8 per group, statistical analysis using Welch’s T-test; B–E Intracellular pH as measured using cSNARF1 in varying extracellular pH, n = 158–438 cells analyzed per group, statistical analysis using Kruskal–Wallis test. *p < 0.05, ***p < 0.001, ****p < 0.0001