Skip to main content
Fig. 4 | BMC Biology

Fig. 4

From: Proton export upregulates aerobic glycolysis

Fig. 4

In vivo CA-IX expressing cell studies on tumor growth, metastasis, and response to buffer therapy. A Primary tumor volume of control or CA-IX cells implanted in the mammary fat pad, n = 10 mice per group, statistical analysis using Welch’s T-test. Statistical analysis of the tumor volume was carried out to determine if tumor growth rate was different between the Mock-2 and M6 groups. Linear regression analysis showed the differences between the slopes were extremely significant. F = 21.03. DFn = 1, DFd = 116, P < 0.0001. B Representative H&E staining of resected primary tumors. M6 CA-IX group showed high infiltration of stromal cells compared to MOCK-2. C Representative whole lung images from MOCK-2 and M6 experimental metastasis model showing the extent of metastasis visible. D Representative immunohistochemistry of H&E staining in the lungs of the experimental metastasis groups, showing gross metastasis in the lungs. E Kaplan–Meier survival curve of experimental metastasis study in SCID beige mice, 90-day endpoint after tail vein injection of cells. Log-rank test performed p = 0.0062, df = 3. N = 5(MCF-7), 6(MOCK-2), 7(M1), 7(M6) mice. F Representative immunohistochemistry of M6 lungs with antibodies towards CA-IX and ER, to confirm the generated M6 clones were the cells forming tumors in the lungs. MCF-7 cells are ER-positive. G Effect of buffer therapy on experimental metastasis of CA-IX clone M6 and MOCK-2 cells in SCID beige mice and the % of tumor burden in the lungs, 77 days after IV injection of cells. Data are shown as average % ± SD, N = 6(M6), 9(M6 Bicarb), 9(MOCK-2), 7(MOCK-2 Bicarb) mice, statistical analysis using ordinary one-way ANOVA. H Immunohistochemistry of H&E staining in the lungs of the M6 control and bicarb-treated experimental metastasis study groups, showing gross metastasis of the lungs. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Back to article page