Fig. 6

In vivo PMA1 expressing cell studies on tumor growth, metastasis, and expression of metabolic markers. A Representative lung images (5 mm and 100 µm) from the experimental metastasis study in SCID beige mice, whereby MOCK-1 or PMA1-C1 cells were injected via the tail vein and allowed to grow for 3 months. At the endpoint lungs were resected and sections were stained with H & E to look for metastases, and PMA1 to confirm expression in the PMA1-C1 metastases. B Primary tumor growth rate in SCID beige mice of MOCK-1 or PMA1-C1 cell lines, injected subcutaneously (n = 7(MOCK-1) n = 6 (PMA1-C1)). C Quantification of primary tumor volume, MOCK-1 or PMA1-C1, resected after 33 days of growth in SCID beige mice. Tumors were resected to allow for spontaneous metastasis studies to continue (n = 9 (MOCK-1) and n = 9 (PMA1)). D Histological grade of MOCK-1 and PMA1 tumors (n = 10 (MOCK-1) and n = 9 (PMA1). E Representative images of H & E staining in MOCK-1 and PMA1 primary tumors were used to score histological grade. F,G Quantification of immunohistochemistry staining and representative images of PMA1 protein in FFPE sections of resected primary tumors, MOCK-1 and PMA1-C1 (N = 10 (MOCK-1) and N = 9 (PMA1)). H,I Quantification of immunohistochemistry staining and representative images of MCT1 protein in FFPE sections of resected primary tumors, MOCK-1 and PMA1-C1 (n = 10 (MOCK-1) and n = 9 (PMA1)). J,K Quantification of immunohistochemistry staining and representative images of CA-IX protein in FFPE sections of resected primary tumors, MOCK-1 and PMA1-C1 (n = 10 (MOCK-1) and n = 9 (PMA1)). Data are shown as mean ± SD. Statistical analysis using unpaired t-test with Welch’s correction *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001