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Fig. 4 | BMC Biology

Fig. 4

From: Transcriptomic evidence for visual adaptation during the aquatic to terrestrial metamorphosis in leopard frogs

Fig. 4

Upregulation of CYP27C1 in tadpoles corresponds to red-shifted absorbance spectra of visual pigments and use of primarily A2 chromophore as detected by microspectrophotometry (MSP). A Plots of normalized read counts of CYP27C1 for light (open) and dark (closed) exposed juveniles and tadpoles. Data are plotted with an additional pseudocount of 0.5 to allow for log plotting. Numbers beside the data points indicate the Gosner stage or size (SVL, mm) of the respective tadpole or juvenile. B Conversion of vitamin A1 chromophore to vitamin A2 chromophore by CYP27C1. Based on Enright et al. [37]. C Representative MSP absorbance spectra from RH1 (red) rod photoreceptors of an adult and a tadpole. Absorbance spectra were found to be wider and best-fit by the A2 Govardovskii et al. [55] template in the tadpole, and narrower and best-fit by the A1 Govardovskii template in the adult. Each absorbance spectra curve is coloured to match the wavelength of light at the corresponding λmax. Normalized count data for each gene are found in Additional file 6. Absorbance spectra for additional photoreceptor cell types and complete results tables can be found in Additional file 1: Figs. S4–S6 and Table S2, and Additional file 7

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