Fig. 3.

Identification of nematode resistance genes in P. kansuensis. a Bulked segregation analysis to locate the nematode resistance gene using a backcross population. b The Indels in the promoter and mRNA regions of one NBS-LRR genes on Chr. 2. “Ins” and “Del” indicate an insertion and deletion, respectively. And the number after the variation type indicates the length (bp) of Indels. c Expression of Prupe.2G053600 in two accessions (“Hong Gen Gan Su Tao 1#” and “Bailey”) inoculated with nematode. e Functional validation of Prupe.2G053600 through analysis of transgenic tomato plants expressing Prupe.2G053600 under nematode treatment. e Promoter activity assay. Promoters with different lengths were fused to the GUS gene in plasmid pBI101. GUS was dyed, and its activity was measured using protein extracts of tobacco. pBI101 and pBI121 indicate negative control and positive control, respectively