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Fig. 6 | BMC Biology

Fig. 6

From: A gossypol derivative effectively protects against Zika and dengue virus infection without toxicity

Fig. 6

Efficacy of gossypol derivative ST087010 in protecting Ifnar1−/− mice from lethal ZIKV challenge. A Schematic diagram of experimental procedures. Mice were intraperitoneally (i.p.) treated with ST087010, gossypol (control), or DMSO (negative control), as shown in the figure. B The treated mice were infected with ZIKV human strain R103451 (200 PFU/mouse), followed by evaluation of survival rate (a) or weight changes (b) for 21 days. The data are expressed as the mean ± s.e.m. of 6 mice in each group. In a separate experiment, ST087010 or gossypol-treated mice were infected with ZIKV human strain PAN2016 (200 PFU/mouse). Five days post-infection (dpi), viral titers were assessed in tissues by plaque assay (C), and ZIKV or caspase-3 signals were measured in the eye (D) and testis (E) tissues by immunofluorescence staining. The data (C) are expressed as the mean ± s.e.m. of 5 mice in each group, and significant differences among mice treated with ST087010, gossypol, and DMSO in the respective tissues (heart, testis, eye, kidney, or brain) were compared and are shown as *, **, and ***. The detection limit is 25 PFU/g. ZIKV (green) and caspase-3 (red) (in D, E) were stained with anti-ZIKV and anti-active caspase-3 antibodies, respectively. Nuclei (blue) were stained with DAPI (4′,6-diamidino-2-phenylindole). Representative images of immunofluorescence staining are shown. Magnification: 63×, and scale bar: 10 μm

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