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Fig. 9 | BMC Biology

Fig. 9

From: A gossypol derivative effectively protects against Zika and dengue virus infection without toxicity

Fig. 9

Efficacy of gossypol derivative ST087010 in protecting Ifnar1−/− mice from DENV-2 challenge. A Schematic diagram of experimental procedures. Mice were i.p. treated with ST087010, gossypol control, or DMSO (negative control) and infected with DENV-2 human strain V594. B Representative images of DENV-2 titers in the brain, kidney, heart, and sera analyzed by flow cytometry at 3 dpi. C6/36 cells with or without DENV-2 infection were used as positive and negative controls, respectively. The percentage of infected cells to the total number of cells is shown in each figure. C Detection of DENV-2 titers in challenged mouse tissues and sera at 3 dpi. Viral titers are expressed as infectious units/g (for the brain, kidney, or heart) or infectious units/ml (for the sera), as assessed by flow cytometry assay (see the “Methods” section) and calculated based on B. Viral titers (infectious units/ml or infectious units/g) were calculated as the formula ((% of infected cells)×(total number of cells)×(dilution factor)/(amount of inoculum added to cells)). Differences among mice treated with ST087010, gossypol, or DMSO in the respective tissues (brain, kidney, and heart) or sera were compared. *, **, and *** indicate significant differences of DENV-2 infection between ST087010 and gossypol or DMSO groups, or between gossypol and DMSO groups. The data are presented as the mean ± s.e.m of 5 mice in each group. The experiments were repeated twice with similar results

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