Fig. 3

ANLN depletion hinders adherens junction assembly in cultured keratinocytes. A shScr- (Ctrl) and shAnln-926-transduced primary mouse keratinocytes (1⁰MKs) were induced to form adherens junctions by switching from low-calcium (50 μM) to high-calcium (1.5 mM) media and then immunolabeled for E-cadherin at the indicated time points. Asterisks denote abnormal junctions. Nuclei were stained with DAPI (blue). Scale bars = 20 μm. B Quantification of adherens junction organization from the data shown in A. Twenty-four hours after calcium switch, Ctrl, n = 35 fields; shAnln-926, n = 23 fields; from three independent experiments. For mature junctions, P = 0.001. For abnormal junctions, P < 0.0001. Forty-eight hours after calcium switch, Ctrl, n = 16 fields; shAnln-926, n = 17 fields; from three independent experiments. For intermediate junctions, P = 0.0259. For mature junctions, P < 0.0001. For abnormal junctions, P < 0.0001. All analyses were by unpaired two-tailed t-test