Fig. 5

ANLN is essential for cytokinesis in cultured cells but not in the epidermis. A Whole-mount immunofluorescence images of control (Ctrl) and shAnln-926-transduced E14.5 and E16.5 embryos immunolabeled for E-cadherin and imaged at the middle of the basal layer. B Quantification of multinucleated cells from the data shown in A. n = 20 and 23 Ctrl and shAnln-926-transduced microscopic fields, respectively, from three E14.5 embryos per condition. n = 21 and 23 Ctrl and shAnln-926 microscopic fields, respectively, from three E16.5 embryos per condition. Horizontal bars represent the mean, and circles represent individual cells. NS denotes not significant. C Wild-type keratinocytes were transduced with shScr (Ctrl) or shAnln-926, cultured in low- (50μM) and high-calcium media (1.5mM), and labeled for F-actin. D Quantification of multinucleated cells from the data shown in C. Low-calcium media: n = 31 and n = 34 ctrl and Anln-926 microscopic fields from three independent experiments. P < 0.0001 by unpaired two-tailed t-test. High-calcium: n = 36 and n = 34 ctrl and Anln-926 microscopic fields; from three independent experiments. P < 0.0001 by unpaired two-tailed t-test. Horizontal bars represent the mean, and circles represent the percentage of multinucleated cells in every microscopic field. Insets in A show the transduced cells (H2B−GFP+). Nuclei were stained with DAPI (grayscale). Scale bars = 20 μm