Fig. 1

VE-1 regulates light-dependent transcription and the accumulation of carotenoids. a Effect of light intensity on the accumulation of carotenoids. Mycelia of the wild-type and Δve-1 mutant strains were grown for 2 days in the dark at 22 °C and illuminated for 2 min prior to incubation for 24 h in the dark at 8 °C. The plot shows the average and standard error for six measurements for each light intensity. b Light-dependent transcription and photoadaptation. Wild-type and Δve-1 mutant strains were grown for 2 days at 22 °C in the dark and then exposed to light during the times indicated prior to RNA purification and quantification by RT-PCR. The plots show the average and standard error of the mean of the relative mRNA accumulation in three independent experiments. The results from each PCR for each gene were normalized to the corresponding PCR for tub-2 to correct for sampling errors. Then, the results were normalized to those obtained with the wild type after exposure to 30 min of light. c Light-dependent phosphorylation of the photoreceptor WC-1. Mycelial samples of the wild-type and the Δve-1 mutant strains were grown for 2 days at 22 °C in the dark and then exposed to light during the times indicated. Total protein extracts were separated by SDS-PAGE, and hybridized with an antibody specific for WC-1. Two hundred micrograms of proteins was loaded per lane. Additional bands are due to the transient light-dependent WC-1 phosphorylation. As loading control, we used a Coomassie staining of each protein sample. d Subcellular localization of VE-1. Mycelial samples of the ve-1^FLAG strain were grown for 2 days at 30 °C in the dark, light, or grown in the dark and exposed to light during 30 min. Total protein samples (T), or samples enriched in cytoplasmic (C) or nuclear (N) proteins were separated by SDS-PAGE, and hybridized with antibodies specific for FLAG or histone H3. Seventy micrograms of proteins was loaded per lane. As loading control, we used a Coomassie staining of each protein sample