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Fig. 2 | BMC Biology

Fig. 2

From: Light regulates the degradation of the regulatory protein VE-1 in the fungus Neurospora crassa

Fig. 2

Light regulates the accumulation of VE-1 during conidiation. a VE-1 regulates the growth of aerial hyphae. Aerial hyphae of the wild-type, Δve-1, Δwc-1, and Δwc-1 Δve-1 strains after 3 days of growth in minimal agar at 30 °C in dark or light. The length of aerial hyphae growing up from vegetative mycelia in two representative cultures is indicated by arrows. b Length of aerial hyphae in each strain after growth at 30 °C in dark or light. Average and standard error of the mean in 3–5 independent experiments. c Accumulation of VE-1 during conidiation. Upper part. Protein samples from cultures kept in the dark or light were obtained from mycelia growing vegetatively in liquid media (M), or as supporting vegetative mycelia after 15 h (M15) or 24 h (M24) of transfer from liquid media to the surface of an agar plate with minimal media to induce conidiation. Conidiating aerial hyphae were collected after 15 h (A15) or 24 h (A24) of the induction of conidiation. We used the ve-1FLAG and the Δwc-1 ve-1FLAG strains. Bottom part. Proteins were separated by SDS-PAGE, and hybridized with an antibody specific for FLAG. One hundred micrograms of proteins was loaded per lane. As loading control, we used a Coomassie staining of each protein sample. d VE-1 accumulates in the nuclear fraction during conidiation. Upper part. We collected samples of the ve-1FLAG strain grown in the light from vegetative mycelia (M), supporting vegetative mycelia (M15) or (M24), and aerial hyphae (A15) or (A24). Bottom part. Total protein samples (T), or samples enriched in cytoplasmic (C) or nuclear (N) proteins were separated by SDS-PAGE, and hybridized with antibodies specific for FLAG or histone H3. Seventy micrograms of proteins was loaded per lane. As loading control, we used a Coomassie staining of each protein sample

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