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Fig. 3 | BMC Biology

Fig. 3

From: Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging

Fig. 3

Deletion of mitochondrial proteases affects longevity. A Scheme and table depicting the localization of the mitochondrial proteases Lon1, Yta12, Yme1, and the adaptor protein Mgr3. B, C Deletion of the mitoproteases Lon1 or Yta12 decreases lifespan, whereas deletion of Mgr3 or Yme1 increases longevity. Strains 972 (WT), lon1Δ, yta12Δ, mgr3Δ, and yme1Δ were grown in rich media with 3% glucose and viability was measured by FACS using propidium iodide (B) and survival spots (C). B Line plot represents the local regression curves for the average survival of each strain (n > 30) at different time points. Each survival curve also displays a 95% confidence interval band. Bar plot depicts the average area under the survival curve of each strain, and error bars represent SD. Significant differences between deletion strains and wild type were determined by two-sided t-test (*p < 0.05, **p < 0.01, ***p < 0.001). C Serial dilutions of culture samples from the logarithmic phase (Log) and days 2, 5, and 6 of the stationary phase were spotted onto rich media plates

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