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Fig. 7 | BMC Biology

Fig. 7

From: Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging

Fig. 7

Mitophagy is required for lifespan extension in the long-lived mutants mgr3Δ and yme1Δ. A Monitoring mitophagy induction in lon1Δ, yta12Δ, mgr3Δ, and yme1Δ strains. The experiment was performed as in Fig. 6A. Samples correspond to cells grown in rich media containing 3% or 1% glucose and day 3 of the stationary phase. Ponceau was used as the loading control. Quantification of mitophagy activation is shown in Fig. S6A. B Mitophagy contributes to the longevity extension observed in mgr3Δ and yme1Δ strains under high glucose conditions (3% Glu). Lifespan of 972 (WT), pka1Δ, mgr3Δ, yme1Δ, atg43-1, mgr3Δ atg43-1, and yme1Δ atg43-1 strains was measured by propidium iodide staining and FACS. Line plot represents the local regression curves for the average survival of each strain (n > 10) at different time points. Each survival curve also displays a 95% confidence interval band. Bar plot depicts the average area under the curve of each strain, and error bars represent SD. Significant differences between deletion strains and wild type, and mgr3Δ and yme1Δ mutants versus mgr3Δ atg43-1 and yme1Δ atg43-1, respectively, were determined by a two-sided t-test (*p < 0.05, **p < 0.01, ***p < 0.001). C Model depicting the effect of deletion of the three mitochondrial proteases and the adaptor protein Mgr3 on longevity. Short-lived mutants, lon1Δ and yta12Δ, are characterized by impaired respiration and loss of mitophagy which curtail cell survival during the stationary phase. Conversely, in long-lived mutants, mgr3Δ and yme1Δ, enhanced respiratory activity together with the elimination of damaged mitochondria by mitophagy contribute to the extension of chronological lifespan

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