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Fig. 7 | BMC Biology

Fig. 7

From: Highly distinct genetic programs for peripheral nervous system formation in chordates

Fig. 7

New vPNS genes are regulated by BMP signaling in ventral ectoderm of P. mammillata embryos. A–F Expression Id.b and Id.c at early neurula stages in control embryos (A, D), following treatment from initial gastrula stage (St 10) with BMP2 protein (B, E) or with DMH1 (C, F). Expression of Dll, Id.e, Ncam, Nherf.a, Phmamm.g00001319 and Selectin in control embryos at mid (G, K, Q, U, Y) or late tailbud stages (I, M, O, S, W, A’), following treatment from the 8-cell stage with BMP2 protein at mid tailbud stages (H, L, R, V, Z) or from initial gastrula stage with DMH1 at late tailbud stages (J, N, P, T, X, B’). Embryos are shown in lateral view with dorsal to the top and anterior to the left except for (D–F) which are in ventral view with anterior to the left and left side to the top. Each experiment has been performed twice. The number of embryos examined for each condition is between 15 and 40, and the pictures shown correspond to the phenotype observed in over 90% of the embryos. Scale bars: 50 μm

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