Fig. 4
Organ-specific expression profile of E1c-transcript (exon 1c). A Graphical illustration of PGC-1α-promoters and corresponding starting exons. The canonical promoter, responsible for exon 1a, lies between the alternative (known) promoter (regulating exons 1b and b’) and the new putative promoter site controlling novel exon 1c. B Novel predicted promoter site associated to exon 1c. Prediction (using ‘ElemeNT’57) of mammalian initiator element (Inr) and corresponding downstream promoter element (DPE) around the transcription start site (marked in grey) of exon 1c. C Expression data of DNA samples derived from qPCR with primers targeting new Exon1c-Exon2-Junction in heart, skeletal muscle (SkM), brown adipose tissue (BAT), white adipose tissue (WAT), kidney, spleen, liver, pancreas, mid-brain and telencephalon (Telenc.), normalized to housekeeper (NUDC) and factorized by 1000 for better visualization. Highest expression can be observed in BAT and muscle tissue (heart, SkM); lower, but detectable, expression levels in kidney, WAT and brain. No detection (n.d.) of the new junction could be seen in liver, pancreas and spleen