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Fig. 5 | BMC Biology

Fig. 5

From: Full-length transcriptomic analysis in murine and human heart reveals diversity of PGC-1α promoters and isoforms regulated distinctly in myocardial ischemia and obesity

Fig. 5

PGC-1α isoform expression in heart in lean or diet-induced obesity (DIO) mice. Housekeeper-normalized expression levels at baseline for either lean mice (black bars) or mice with diet-induced obesity (DIO, grey bars). Shown is isoform expression for PGC-1α starting exons (A), for the four group-wise testable isoforms (B) and for the four single-detectable isoforms (C). A Isoform expression by promoter usage. While expression levels for exon 1a and exon 1c are similar between both conditions, transcripts containing exon 1b and exon 1b’ are significantly lower expressed in DIO. B Expression of group-wise detectable isoforms. While isoforms with novel C-terminal end are unchanged between both diets, the other group-wise detectable isoforms show significantly lower expression in DIO. C Expression for isoforms which are single-detectable through PCR. While CT-PGC-1α-E3c is equally expressed under both conditions, the other single-detectable isoforms show significantly reduced expression in DIO. Data acquired by qPCR using cDNA using primers covering specific starting exon 1 (a, b, b’ or c) and exon 2 resp. using primers covering specific exon-exon junctions. Expression values are normalized to housekeeper NUDC. n = 4 each, bars depict mean values, error bars represent SD. Significance calculated by unpaired Student’s t test (ns p > 0.05, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001)

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