Table 2 List and sequences of primers used for scRNAseq library construction
From: Evidence for close molecular proximity between reverting and undifferentiated cells
Primer name | 5′ to 3′ |
|---|---|
Index_RT_primers (cell BC and UMI) | 5′-CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACG TGTGCTCTTCCGATCTXXXXXXNNNNNNNNTTTTTTTTTTTTTTTTTTTTV-3′ |
P5N6_XXXX (Plate BC) | 5′-CTACACGACGCTCTTCCGATCTXXXXNNNNNN-3′ |
P5.rd1 | 5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCC CTACACGACGCTCTTCCGATCT-3′ |
P7.rd2 | 5′-CAAGCAGAAGACGGCATACGAGAT GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3′ |