Fig. 6

Interactions between CRS and cytokinesis regulators. A Immunoprecipitation of protein extracts from cytoplasmic fraction of telophase synchronized HeLa cells (S2 fraction). IP sample immunoprecipitated with BAF53a or Tip60 antibodies (+anti-BAF53a; +anti-Tip60) were compared to negative controls (−anti-BAF53a or −anti-Tip60). Anillin, Aurora B, CEP55, CIT-K, MKLP2, MRG15, spastin, and α-tubulin were found in the IP samples immunoprecipitated with BAF53a or Tip60 antibodies, but not in the negative controls. Three independent IP experiments were performed. IN = input, IP = immunoprecipitation. Synchronization of the HeLa cells was performed according to Messina et al. [31]. B Histograms showing the quantitative analysis of mislocalizations of cytokinesis regulators at the midbody in mocks and BAF53a or Tip60 depleted cells. Three independent experiments were performed and at least 300 telophases were scored in both RNAi-treated and control cells. *=P<0.05,**=P<0.005, ***=P<0.0005. C Examples of mislocalization of cytokinesis regulators in mock and BAF53a or Tip60 depleted cells. From left to the right: DAPI (blue), anti-α-tubulin (green), cytokinesis regulators (red), and merge. Scale bar = 10 μm