Table 2 Primers for RT-qPCR
Gene target | Forward primer (5′-3′) | Reverse primer (5′-3′) | Amplicon length (bp) | Transcript variants amplified | NCBI accession ID of first variant |
|---|---|---|---|---|---|
Human | |||||
 TUG1 | AGCGTGGGTGTACGTAAAGG | CCAAGGATTGGGGAACTGCT | 82 | 1,2,3,6,7,8 | NR_110492.1 |
 PPARGC1A | TGAGAGGGCCAAGCAAAG | ATAAATCACACGGCGCTCTT | 64 | 1-7 | >NM_001330751.2 |
 TFAM | GAACAACTACCCATATTTAAAGCTCA | GAATCAGGAAGTTCCCTCCA | 95 | 1,2 | >NM_003201.3 |
 GAPDH | AGCCACATCGCTCAGACAC | GCCCAATACGACCAAATCC | 66 | 1,3,4,7 | >NM_002046.7 |
Mouse | |||||
 Tug1 | GAGACACGACTCACCAAGCA | GAAGGTCATTGGCAGGTCCA | 165 | a,b,c | >NR_002321 |
 Ppargc1a | TCCTCTTCAAGATCCTGTTAC | CACATACAAGGGAGAATTGC | 78 | 1-9 | >NM_008904.2 |
 Nrf1 | AAACAAAGGGTTTCATGGAC | GGTACGAGATGAGCTATACTG | 149 | 1-10 | >NM_001164226.1 |
 Gabpa (Nrf2) | CAAGTATTGACAGTACCAGC | CTCTCTCTTCAATTTCTGCAC | 127 | 1,4,5,9 | >NM_207669.2 |
 Myod | CTGGTTCTTCACGCCCAAA | CTGGAAGAACGGCTTCGAAGG | 74 | 1 | >NM_010866.2 |
 Myog | GGGGCAATGCACTGGAGTTC | GCAGATTGTGGGCGTCTGTA | 78 | 1 | >NM_031189.2 |
 Gapdh | GCCTGGAGAAACCTGCCAA | CGAAGGTGGAAGAGTGGGAG | 144 | 1,3 | >NM_001289726.1 |