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Fig. 4 | BMC Biology

Fig. 4

From: The testis-specific E3 ubiquitin ligase RNF133 is required for fecundity in mice

Fig. 4

Rnf133 KO and Rnf133/148 DKO sperm show abnormal morphology that correlates with immotility. A Flagellar waveform patterns of Rnf133 HET and KO, and Rnf133/148 DKO sperm. n = 3 mice/genotype. Black lines indicate traces from motile sperm. The representative images of Rnf133 HET and KO and Rnf133/148 DKO sperm are shown at the bottom. B Flagellar waveform patterns of abnormal sperm from Rnf133 KO and Rnf133/148 DKO. n = 3 mice/genotype. Blue and orange lines indicate sperm bent at the principal piece and at the neck, respectively. C Average percentages of motile and immotile sperm with normal or abnormal morphology are presented for the Rnf133 HET, Rnf133 KO, and Rnf133/148 DKO mice. n = 3 mice/genotype. D Representative multichannel fluorescence staining of Rnf133 HET and KO sperm incubated with membrane-permeant SYBR14 (green) and membrane-impermeant propidium iodide (PI; red) nucleic acid stains to identify live (SYBR14 + /PI −) and dead (SYBR-/PI +) sperm. n = 4 mice/genotype. E Results from flow cytometry-based quantification of the percentage of live (SYBR14 + /PI − and SYBR + /PI +) and dead (SYBR − /PI +) Rnf133 HET and KO sperm. SYBR + /PI + cells mark a population of cells that were alive when removed from the animal but are rapidly undergoing death in vitro. n = 4 mice/genotype, and the data are expressed as the mean ± SEM

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