Fig. 4
From: Kinesin-3 motors are fine-tuned at the molecular level to endow distinct mechanical outputs
Kinesin-3 motors glide microtubules faster. Collective motor properties of kinesin-3 motors in MT gliding assays. A Schematic representation of MT gliding assay. C-terminal mCitrine-tagged truncated constitutively active kinesin-3 motors were attached to a glass surface coated with GFP nanobodies. Subsequently, fluorescently labeled MTs were introduced into the flow chamber. B–F Histograms showing velocity of MT sliding by kinesin-1 motor B KHC (1–560) and kinesin-3 motors C KIF1A(1-393LZ), D KIF13A(1-411ΔP), E KIF13B(1-412ΔP), and F KIF16B(1–400) were plotted and fit to Gaussian distribution. Average gliding velocities are indicated as mean ± SEM on top-right or left corner. N, number of motility events analyzed across three independent experiments. G Gliding velocities for kinesin-1 and kinesin-3 motors were plotted as mean ± SEM