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Table 1 Biochemical and biophysical properties of kinesin-3 family motors

From: Kinesin-3 motors are fine-tuned at the molecular level to endow distinct mechanical outputs

Single-molecule motility assay

Motor

Velocity (μm s−1)

Run length (μm)

Estimated stepping rate (s−1)

Mean run time (s)

Detachment rate (s−1)

KIF1A(1-393LZ)

2.41 ± 0.02

10.56 ± 0.26

301.29

4.38 ± 1.3

0.23 ± 0.05

KIF13A(1-411ΔP)

1.55 ± 0.02

10.24 ± 0.22

194.19

6.61 ± 2.6

0.15 ± 0.07

KIF13B(1-412ΔP)

1.36 ± 0.01

10.96 ± 0.22

170.16

8.06 ± 2.4

0.12 ± 0.09

KIF16B(1–400)

0.95 ± 0.01

9.34 ± 0.22

118.75

9.83 ± 1.2

0.10 ± 0.02

KHC(1–560)

0.82 ± 0.01

1.14 ± 0.04

102.15

1.39 ± 0.3

0.72 ± 0.05

ATPase assay

Motor

kcat (s−1)

Km (µM)

Basal activity (s−1)

Step size (nm)

 

KIF1A(1-393LZ)

290.9 ± 8.69

7.03 ± 0.79

0.08

8.28

 

KIF13A(1-411ΔP)

181.7 ± 8.31

5.16 ± 0.84

0.014

8.53

 

KIF13B(1-412ΔP)

171.8 ± 7.82

4.23 ± 0.74

0.08

7.91

 

KIF16B(1–400)

124.7 ± 6.94

2.43 ± 0.58

0.32

7.61

 

KHC(1–560)

95.44 ± 15.5

10.23 ± 3.63

0.044

8.54

 

Microtubule gliding assay

Motor

Gliding velocity (μm s−1)

    

KIF1A(1-393LZ)

1.16 ± 0.14

    

KIF13A(1-411ΔP)

0.58 ± 0.05

    

KIF13B(1-412ΔP)

0.60 ± 0.06

    

KIF16B(1–400)

0.48 ± 0.08

    

KHC(1–560)

0.46 ± 0.06

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