From: Kinesin-3 motors are fine-tuned at the molecular level to endow distinct mechanical outputs
Single-molecule motility assay | |||||
Motor | Velocity (μm s−1) | Run length (μm) | Estimated stepping rate (s−1) | Mean run time (s) | Detachment rate (s−1) |
KIF1A(1-393LZ) | 2.41 ± 0.02 | 10.56 ± 0.26 | 301.29 | 4.38 ± 1.3 | 0.23 ± 0.05 |
KIF13A(1-411ΔP) | 1.55 ± 0.02 | 10.24 ± 0.22 | 194.19 | 6.61 ± 2.6 | 0.15 ± 0.07 |
KIF13B(1-412ΔP) | 1.36 ± 0.01 | 10.96 ± 0.22 | 170.16 | 8.06 ± 2.4 | 0.12 ± 0.09 |
KIF16B(1–400) | 0.95 ± 0.01 | 9.34 ± 0.22 | 118.75 | 9.83 ± 1.2 | 0.10 ± 0.02 |
KHC(1–560) | 0.82 ± 0.01 | 1.14 ± 0.04 | 102.15 | 1.39 ± 0.3 | 0.72 ± 0.05 |
ATPase assay | |||||
Motor | kcat (s−1) | Km (µM) | Basal activity (s−1) | Step size (nm) |  |
KIF1A(1-393LZ) | 290.9 ± 8.69 | 7.03 ± 0.79 | 0.08 | 8.28 |  |
KIF13A(1-411ΔP) | 181.7 ± 8.31 | 5.16 ± 0.84 | 0.014 | 8.53 |  |
KIF13B(1-412ΔP) | 171.8 ± 7.82 | 4.23 ± 0.74 | 0.08 | 7.91 |  |
KIF16B(1–400) | 124.7 ± 6.94 | 2.43 ± 0.58 | 0.32 | 7.61 |  |
KHC(1–560) | 95.44 ± 15.5 | 10.23 ± 3.63 | 0.044 | 8.54 |  |
Microtubule gliding assay | |||||
Motor | Gliding velocity (μm s−1) |  |  |  |  |
KIF1A(1-393LZ) | 1.16 ± 0.14 |  |  |  |  |
KIF13A(1-411ΔP) | 0.58 ± 0.05 |  |  |  |  |
KIF13B(1-412ΔP) | 0.60 ± 0.06 |  |  |  |  |
KIF16B(1–400) | 0.48 ± 0.08 |  |  |  |  |
KHC(1–560) | 0.46 ± 0.06 |  |  |  |  |