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Fig. 9 | BMC Biology

Fig. 9

From: The protein aggregation inhibitor YAT2150 has potent antimalarial activity in Plasmodium falciparum in vitro cultures

Fig. 9

Analysis of the effect of YAT2150 on markers of protein aggregation in live P. falciparum cultures. A,B Western blot assays for the detection of ubiquitinated proteins in cultures treated for 0.5 to 24 h with 90 nM YAT2150. Panel B corresponds to an untreated control. Anti-spectrin antibody is used as loading control shown at the bottom of each gel. C ThT fluorescence of P. falciparum culture extracts normalized to have equal protein content, either non-treated or treated with YAT2150 at its in vitro IC10 (27 nM) and IC50 (90 nM), for 90 min and 4 h. A non-parasitized RBC protein extract is shown as reference. The mean fluorescence intensity value of each sample in each wavelength is represented. D Western blot assays for the detection of ubiquitinated proteins in cultures treated for 90 min with different concentrations of YAT2150. E Dot blot assay of the same samples from panel D using an antibody against amyloid fibrils. F Isobologram of the interaction between YAT2150 (Y) and artemisinin (A) at different Y:A ratios

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