Fig. 2

Hmga2 association with chromatin. A Comparison of the expression profiles of wt and Hmga2 KO cells induced to differentiate into EpiLCs. The profiles of the two cell types are similar (see also Additional file 2: Table S1) with some differences indicated in the Venn diagram. The heatmap shows the 2634 genes with a common behavior in the three cell clones, according to the Venn diagram. B DNA sequence motifs in the regions of the peaks of Chip-seq experiment with chromatin from wt EpiSC, immunoprecipitated with the anti-Hmga2 antibody and analyzed by MEME-chip software with default options. The three consensus sequences emerging from the analysis are shown. C Venn diagram of the alignment between Hmga2 peaks and those of chromatin markers H3K4me1 (GSM1382217 from ref. [19]) and H3K27ac (GSM1382219 from ref. [19]). D ChIP-seq data of Hmga2 in wt EpiSCs were aligned with available data of DamID in mouse ESCs (ref. [20], GSE17051). The results show a significantly overlap with the regions associated with Lamin b1 (about 51% Spearman correlation). The image shows a 196 Mb region of chromosome 1