Fig. 5

Mislocalization of H3K9me3 histone mark in Hmga2 KO and KD cells upon EpiLC induction. A Immunoflurescence experiments on Hmga2 wt and KO cells at day 1 after the induction of EpiLCs of Lmnb1 (red) and H3K9me3 (green). B Lmnb1 (red) and H3K9me3 (green) immunofluorescence on Hmga2 KD cells (day 3 after the induction). Non-silencing siRNA was used as control. In all immunofluorescence experiments, DAPI (blue) was used to counterstain the nuclei. All pictures are shown as single z-plane, ROI 1024×1024, scale bar= 50μm. Quantification graphs show the percentage of Hmga2 wt and KO cells with H3K9me3 mislocalization (n≥200 nuclei per condition) and the percentage of Hmga2 KD cells showing H3K9me3 mis-localization (n≥ 300 nuclei per condition). The count for Hmga2 wt and KO cells was performed at day 1 of differentiation, while the count for of Hmga2 KD cells was performed at day 3 of EpiLC transition, on three biological replicates for each condition. Error bars represent standard deviation. Statistical significance was determined using Student’s t-test (ns: not significant, ***p<0.001)