Fig. 1

SP140 expression associates with inflammatory diseases and mucosal macrophages of CD patients. a SP140 gene expression in human tissues and cell types as indicated. Expression is given as count normalized with MAS5.0. b SP140 gene expression in white blood cells of normal healthy controls (N) (n=33), Crohn’s disease (CD) (n=6), ulcerative colitis (UC) (n=6), systemic lupus erythematosus (SLE) (n=64), chronic lymphocytic leukemia (CCL) (n=21), acute myeloid leukemia (AML) (n=7), and rheumatoid arthritis (RA) (n=32) patients. c SP140 gene expression in human colon tissue obtained from N (n=18), non-inflamed and inflamed CD (n=5 and 13, respectively), and UC (n=3 and 17, respectively) colonic tissues. Data was collected from in-house GSK microarray profiler. Expression is given as count normalized with MAS5.0. d Immunohistochemistry of SP140 protein in colon tissue obtained from N and inflamed CD and inflamed UC tissue, scale bar: 100 μm. e Immunofluorescence staining of DAPI (blue), CD68 (green), and SP140 (red) in N or inflamed CD colon tissue, scale bar: 100 μm. f The average of mucosal cell count per 3 visual fields of total CD68⁺ macrophages, SP140⁺ CD68⁺ macrophages and SP140⁻ CD68⁺ macrophages in N and inflamed CD tissue (n = 3 patients per group). g Immunofluorescence staining of DAPI (blue), HLA-DR (green), and SP140 (red) in uninflamed or inflamed CD colon tissue, scale bar: 100 μm. h The average of mucosal cell count of 3 visual fields per tissue of total HLA-DR⁺ macrophages, SP140⁺ HLA-DR⁺ macrophages, and SP140⁻ HLA-DR⁺ macrophages in N and inflamed CD tissue (n=3 patients per group). Statistical significance is indicated as follows: *P < 0.05, **P < 0.01, ****P < 0.0001