Fig. 7

SP140 preferentially controls the expression of specific gene sets involved in the innate immune response. a Heatmap of the top 100 DEGs and b volcano plots of the all genes comparing 0.1% DMSO- with 0.04 μM GSK761-treated “M1” macrophages after 4 h of 100 ng/mL LPS stimulation. c R2 TSS plot comparing a global differentially SP140-bound genes (DBGs) after 1 h of 100 ng/mL LPS stimulation of 0.1% DMSO- and 0.04 μM GSK761-treated “M1” macrophages. d SP140 ChIP-seq genome browser view of some of the most affected DBGs; TNF, TRAF1, IRF1, and TRAFAIP2. Y axis represents a signal score of recovered sequences in 0.1% DMSO and 0.04 μM GSK761 treated macrophages after 0, 1, and 4 h of 100 ng/mL LPS stimulation. e RNA sequencing-derived gene expression of some of the most DBGs. f Comparative analyses of the top 1000 DBGs (signal) with their gene expression (Wald statistic). Gene expression at 4 h and ChIP at 1 h (left) and gene expression at 4 h and ChIP at 4 h (right), Y axis represents the SP140 differential binding signal (BD). g Homer Known Motif Enrichment using TF motifs and their respective p-value scoring (top) and Homer de novo Motif results with best match TFs (bottom) in 0.1% DMSO-treated “M1” macrophages after 1 h of 100 ng/mL LPS stimulation. h An enrichment analysis targeted chemokine activity for SP140 ChIP-seq (top) and RNA-seq (bottom) comparing 0.1% DMSO- with 0.04 μM GSK761-treated “M1” macrophages at 0, 1, and 4 h of 100 ng/mL LPS stimulation