Fig. 4From: Combined protein and transcript single-cell RNA sequencing in human peripheral blood mononuclear cellsSignificantly differentially expressed genes of cells in each cluster. The expression of 485 transcripts was determined by targeted amplification (BD Rhapsody system). Significant genes defined as adjusted p < 0.05 and log2 fold change > 0. Dot plot: fraction of cells in cluster expressing each gene shown by size of circle and level of expression shown from white (=0) to dark blue (=max, log2 scale). Red bars indicate genes that were significantly higher in one cluster compared to all other clusters of the parent cell type. There were no DEGs in NK or B cell clusters. A CD4+ T cells, B CD8+ T cells, and C monocytes. CM, classical monocytes; INT, intermediate monocytes; NCM, nonclassical monocytes. To denote each of the clusters, the letter “C” is used in CD4+ T and CD8+ T followed by the cluster number. In the case of monocytes, clusters are represented as INT, CM, or NCM followed by the cluster numberBack to article page