Fig. 1
From: NF90 interacts with components of RISC and modulates association of Ago2 with mRNA
NF90 interacts with proteins involved in translational repression and RNA processing. A Representative gene ontology pathways (biological pathways) of NF90-associated proteins (n = 209; 3 or more peptides, FC>2) identified by mass spectrometry. B Cytoplasmic extracts from HEK-293T cells were used for immunoprecipitation using an anti-IgG control or anti-NF90 antibody followed by RNAse A/T treatment or mock treatment, as indicated. Immunoprecipitates and an aliquot of extract (In) were analyzed by western blot, using the indicated antibodies. C FLAG immunoprecipitates of cytoplasmic extracts of NF90-FH overexpressing HEK293T cells were separated by glycerol gradient sedimentation. NF90-containing fractions were analyzed by western blot using the antibodies indicated