Fig. 5
From: NF90 interacts with components of RISC and modulates association of Ago2 with mRNA
Downregulation of NF90/NF45 increases Ago2 binding to selected target mRNAs. RIP analysis of HEK293T cells transfected with NF90/NF45-targeting siRNAs or a non-targeting control (Scr), as indicated. RIPs were performed using anti-Ago2 or a control IgG antibody. Immunoprecipitates were analyzed using RT-qPCR. ND indicates ‘Not Detected’. Data represent mean ± SEM obtained from 3 independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001, NS indicates not significant, independent Student’s t test) (Additional file 9)