Fig. 1

The CoREST complex is present in Nematostella. A Phylogenetic tree showing the presence/absence of Lsd1, HDAC1/2, and CoREST orthologs in the different groups. The tree on the left shows the relationships between the different clades analyzed. Green circles indicate the presence of orthologs within that group while red circles indicate their absence. A yellow circle indicates the groups where we see a CoREST-like protein containing only a single SANT domain. On the right are two representative protein sequences from Nematostella vectencis and Ichthyophonus hoferi. B Dot plot showing proteins derived from a comparison of Co-IP with GFP-Trap beads from NvLsd1^GFP and wild-type planula. Data is derived from three independent biological replicates. Only proteins with p-value < 0.05 (Student’s t-test, two-tailed) are shown. Fold change (FC) in the NvLsd^GFP sample over wildtype is shown on the x-axis and p-value on the y-axis. The four proteins with the highest FC are annotated with names. C Schematic representation of the human CoREST complex as first identified [15] D Table showing the fold change and p-value for the five most enriched proteins in the NvLsd^GFP sample over wildtype. * We did not calculate a p-value for NvPHF21a-like due to a missing value in one of the control samples. E Co-IPs from NvLsd1^GFP planula with either anti-GFP Trap beads or control agarose beads followed by western blot analysis with indicated antibodies. This was repeated 3 times independently with the same result