Fig. 2

NvCoREST is ubiquitously expressed throughout development. A–D Confocal images of immunofluorescence staining showing NvCoREST localization throughout development. Stages are shown on top. E Close up of the ectoderm at planula stage showing colocalization of NvLsd1-GFP and NvCoREST. NvCoREST is shown in magenta, DNA in blue, and NvLsd1-GFP in green. All stainings were performed at least two times independently with a minimum of 10 embryos imaged per stage, per replicate with the same results. Scale bars: 50 µm (A–D) and 20 µm (E)