Skip to main content
Fig. 4 | BMC Biology

Fig. 4

From: A developmental role for the chromatin-regulating CoREST complex in the cnidarian Nematostella vectensis

Fig. 4

NvCoREST is required for normal development in Nematostella. A Schematic showing the intron–exon structure of NvCoREST, the location of the alternatively spliced exon, and indicating both the position and sequence of mutations in NvCoREST Mutant 1 and Mutant 2 lines. B Diagram representing the domain architecture of the NvCoREST long and short isoforms and the truncated products predicted based on the mutations. C–F Live images of primary polyp stage animals generated from in-crosses of heterozygous NvCoREST Mutant 1 or Mutant 2 animals, separated based on size phenotype (indicated on top). Below the images is sequencing data showing the number of animals with the indicated genotype found via sequencing within these pools of animals. Numbers are combined data from 4 independent replicates. G–J Confocal images of representative animals from each phenotypic group stained with phalloidin for F-actin (magenta) and DNA (blue). Stainings were performed three times, independently with a minimum of 10 embryos imaged per condition, per replicate with the same results. Scale bars: 200 µm (C–F) and 50 µm (G–J)

Back to article page